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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Plast Reconstr Surg. 2014 Feb;133(2):301–310. doi: 10.1097/01.prs.0000436840.69752.7e

Figure 1. FLT-4-LacZ mice can be used as an inducible lymphatic reporter mouse.

Figure 1

(A) Schematic figure of FLT-4Cre/LacZ LoxP mice. Treatment with tamoxifen activates LacZ expression and enables visualization of lymphatic vessels with B-gal.

(B) High level activation of LacZ expression in LYVE-1 positive lymphatics is inducible with tamoxifen.

(C) Representative high power (40×) images of skin sections double stained for B-galactosidase (blue) and LYVE-1 (brown stain, left panel) or Von-Willebrand Factor (VWF; brown stain, right panel) demonstrate specificity of Lac-Z expression only in lymphatics vessels (blue/brown double arrow on the left). Notice no overlap with VWF (separate brown and blue arrows) on the right panel.