Figure 7.

Decreased spontaneous activity accompanied by mitochondrial dysfunction in skeletal muscle of high-fat–fed tg^sm/p22phox mice. A: Spontaneous activity was monitored over 8 weeks of high-fat diet feeding and progressively declined in the tg^sm/p22phox mice while remaining constant in the WT mice. B: Mitochondrial fraction from skeletal muscle was prepared as described in Research Design and Methods and subjected to superoxide detection using electron spin resonance. Mitochondrial superoxide production from high-fat diet–fed tg^sm/p22phox mice was increased more than threefold compared with WT controls fed high-fat diet. C: Calcium-induced swelling of skeletal muscle mitochondria was significantly augmented in high-fat diet–fed tg^sm/p22phox mice compared with WT controls fed high-fat diet. n = 11–13. Data are presented as mean ± SEM.