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. 2014 Jun 14;63(7):2464–2473. doi: 10.2337/db13-0835

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© 2014 by the American Diabetes Association.

Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. See http://creativecommons.org/licenses/by-nc-nd/3.0/ for details.

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A: Effects of BF175 on lipid accumulation in Drosophila larvae as detected by Oil Red O staining followed by isopropanol extraction. B–E: C57BL/6J mice were first fed with HFD for 4 weeks and then treated with BF175 (0.3 mg/g body weight per week) or buffer (containing 0.5% DMSO) for a total of 7 days by subcutaneously implanted osmotic pumps. All mice were continuously provided with HFD during the treatment. At the end of the treatment, mice were killed and the livers were collected. B: Effects of BF175 on the triglyceride levels in the mouse liver. C and E: Relative mRNA levels of the indicated genes as detected by qRT-PCR in the mouse liver. D: Immunoblots of hepatic SREBP-1 proteins. Three representative mice from each group are shown. Data are the mean ± SD (n = 6). *P < 0.05; #P < 0.01 vs. control (DMSO).