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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: J Neurochem. 2014 Feb 9;129(6):932–939. doi: 10.1111/jnc.12660

Figure 1.

Figure 1

Primary astrocytes were purified and induced to express mutant human TDP-43. (a-f) Double-labeling fluorescent staining revealed that unpurified glial culture contained astrocytes (GFAP), microglia (Iba-1), and oligodendrocytes (APC). Primary glia culture was isolated from the cortex of GFAP-tTA#2/TRE-TDP43M337V transgenic rats at 3 days of age. (g–l) Immunostaining showed that microglia and oligodendrocytes were eliminated from primary astrocyte culture after three cycles of shaking. (m–o) Immunofluorescent staining revealed that mutant human TDP-43 (hTDP43) was robustly expressed in purified astrocytes. Purified astrocytes were plated on 6-well plates and deprived of Dox on day 2 after plating. All scale bars: 30 μm (a-o).