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. 2014 Jun 15;28(12):1337–1350. doi: 10.1101/gad.240416.114

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© 2014 Liu et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 4. — Pax5 restoration triggers leukemia differentiation and regression. (A) Flow cytometry of CD19 and IgM expression in peripheral blood of wild-type (top panel) and untransplanted Rag1^−/− (bottom panel) control mice. (B) Flow cytometry of CD19 and IgM expression on mononuclear cells from the peripheral blood of a representative Rag1^−/− mouse transplanted with STAT5-CA;Vav-tTA;TRE-GFP-shPax5 leukemia and Dox-treated upon leukemia development as indicated. (C) Time course analysis as shown in B for an independent leukemic recipient mouse. (D) Leukemia burden (proportion of CD19⁺ cells in the blood) upon Dox treatment (mean ± SEM; n = 3 mice). (E) Proportion of CD19⁺ cells coexpressing IgM upon Dox treatment (mean ± SEM; n = 3 mice). (F) Flow cytometry of CD19 and CD93 expression on bone marrow cells (top panels) and splenocytes (bottom panels) isolated from representative leukemic Rag1^−/− mice at various times during Dox treatment as indicated. The left panels are profiles from untransplanted Rag1^−/− control mice, indicating a small population of CD19⁺ pro-B cells in the bone marrow. (G) Flow cytometry of CD19 and IgM expression as shown in F.