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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: J Endocrinol. 2014 Jul;222(1):27–41. doi: 10.1530/JOE-13-0455

Figure 2.

Figure 2

STAT3 phosphorylation following a single i.c.v. injection of ACSF (open bars) or 40 ng of leptin in rats administered control vector (solid bars), rAAV-leptin antagonist into the right MBH (hatched bars) or rAAV-leptin antagonist into the VTA (striped bars) 20 days earlier. STAT3 phosphorylation was assessed 1 hour later in the right MBH (Top) or left MBH (Bottom), and expressed as the ratio of STAT3 Phosphorylation to G3PDH.

Values represent the mean ± SE of 6-8 rats per group. Western analysis required two gels. Samples from Control-Leptin groups were present on both gels and used for normalization across gels. The value of ACSF injected control for each individual tissue is arbitrarily set to 100 with SE adjusted proportionally with remaining groups normalized to the level in ACSF injected control. Top: P< 0.001 for difference with treatment by one-way ANOVA. *P<0.01 for difference between AAV-Control with leptin injection and all other groups by Newman-Keuls post-hoc analysis. Bottom: *P<0.025 for difference with treatment by one-way ANOVA. *P<0.05 for difference between rAAV-Control with ACSF injection and all other groups by Newman-Keuls post-hoc analysis.