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. Author manuscript; available in PMC: 2014 Jun 23.
Published in final edited form as: Stem Cells. 2008 Jun 26;26(10):2735–2745. doi: 10.1634/stemcells.2008-0212

Figure 1.

Figure 1

Adipose stem cell (ASC)-binding Peps identify potential domains of SPARC-cell interaction. (A): Binding of phage to human ASC in individual rounds of a random Pep library selection. Relative phage recovery in subsequent rounds of selection reveals a progressive increase in phage binding. For the insertless phage (fd-tet), used as a negative control for each round of selection, mean binding is shown. (B): Human ASC binding of hPep-phage and mPep-phage. In (A, B), the ratio of phage TU bound over TU applied to cells is plotted. Negative controls: fd-tet and phage displaying an unrelated (control) Pep sequence. Error bars: SEM (%) from the three rounds (A) or from two independent experiments (B). (C): Amino acid sequences of Pep selected on human ASC (green underline) and mouse 3T3–L1 cells (orange underline) matched to the sequence of human SPARC (signal Pep sequence not included). Previously characterized domains of SPARC are indicated; pep4.2 and pep2.1 sequences are highlighted in yellow. Pep similarity criteria: at least three amino acids identical (red) and at least one similar (blue) to the correspondingly positioned SPARC amino acids. Abbreviations: E-C, extracellular Ca(2+)-binding domain; Pep, peptide.