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. 2014 Jun 2;3:e02501. doi: 10.7554/eLife.02501

Figure 1. Chromosomal editing mutants of E. coli and S. cerevisiae.

Figure 1.

(A) Post–transfer hydrolysis of [14C]- Tyr-tRNAPhe (1 µM) by cell-free extracts isolated from wild type (●) and pheT(G318W) (■) E. coli strains (140 mg/ml total protein concentration) or buffer (▲) at 37°C. (B) Posttransfer editing activity of βD243A ctPheRS in S. cerevisiae. Reactions were performed at 37°C with 2 μM Tyr-tRNAPhe and S. cerevisiae wild type FRS1 or frs1-1 (D243A) cell-free extracts normalized to aminoacylation activity (Reynolds et al., 2010). Data points are the mean of at least three independent experiments, with errors bars representing ±1 SD.

DOI: http://dx.doi.org/10.7554/eLife.02501.003