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. 2014 Jun 2;3:e02501. doi: 10.7554/eLife.02501

Figure 6. Incorporation of m-Tyr into the proteome of E. coli.

(A) In vitro 70S ribosomal di-peptide synthesis with either Phe-tRNAPhe or m-Tyr-tRNAPhe (B) LC-MS/MS-MRM quantification of m-Tyr and Phe in protein hydrolysis isolated from E. coli expressed as molar ratio of m-Tyr to Phe. Wild type (Wt) and pheT(G318W) strains grown in M9 minimal media alone and supplemented with m-Tyr are shown. Error bars represent ± standard error of means.

DOI: http://dx.doi.org/10.7554/eLife.02501.012

Figure 6.

Figure 6—figure supplement 1. p-Tyr is not misincorported in the proteome of E. coli at Phe codons.

Figure 6—figure supplement 1.

Mass spectroscopy quantification of p-Tyr and Phe in protein hydrolysis isolated from E. coli expressed as molar ratio of p-tyr to Phe. Wild type and pheT(G318W) strains grown in in M9 minimal media alone and supplemented with p-Tyr are shown. Error bars represent ± standard error of means.
DOI: http://dx.doi.org/10.7554/eLife.02501.013
Figure 6—figure supplement 2. E. coli TyrRS uses m-Tyr.

Figure 6—figure supplement 2.

Aminoacylation of E. coli [32P]-tRNAPhe transcript (0.5 μM) with m-Tyr (1 mM) by E. coli TyrRS (50 nM) at 25°C.
DOI: http://dx.doi.org/10.7554/eLife.02501.014