Table 1.

Amino acid pools in wild type and editing defective E. coli strains

DOI: http://dx.doi.org/10.7554/eLife.02501.007

Strain	Supplement	m-Tyr (µM)*	p-Tyr (µM)	Phe (µM)	p-Tyr/Phe	m-Tyr/Phe
Wild type	+ m-Tyr	2.9 ± 0.06	0.56 ± 0.1	0.63 ± 0.2	0.9 ± 0.0	5 ± 1
pheT(G318W)	+ m-Tyr	2.7 ± 0.5	0.46 ± 0.02	0.90 ± 0.2	0.9 ± 0.2	6 ± 1
Wild type	+ p-Tyr	ND	11 ± 4	0.91 ± 0.1	12 ± 4	ND†
pheT(G318W)	+ p-Tyr	ND	8.9 ± 0.4	0.93 ± 0.1	9.7 ± 1	ND

^*Concentrations of intracellular Phe and Tyr isomers isolated from wild type and pheT(G318W) E. coli strains grown in M9 minimal media supplemented with either m-Tyr or p-Tyr.

^†ND indicates concentrations were below the detectable limit (0.01 µM).