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Steady-state kinetic constants for amino acid activation by PheRS from E. coli and S. cerevisiae cytoplasmic PheRS
| Phe | m-Tyr | p-Tyr | Specificity (kcat/KM/kcat/KM) | ||||||
|---|---|---|---|---|---|---|---|---|---|
| PheRS | KM (µM) | kcat (s−1) | kcat/KM (s−1/µM) | KM (µM) | kcat (s−1) | kcat/KM (s−1/µM) | kcat/KM (s−1/µM) | Phe/m-Tyr | Phe/p-Tyr |
| E. coli | 18 ± 4 | 5.2 ± 2 | 0.29 | 247 ± 60 | 2.1 ± 0.8 | 0.008 | 1.1 × 10-4 | 35 | 2650 |
| Yeast ct | 16 ± 2 | 26 ± 4 | 1.6 | 1150 ± 230 | 26 ± 4 | 0.023 | 0.014 | 71 | 120 |
