Precision-cut lung slices from wild-type (WT, Prkce+/+) and knock-out (KO, Prkce−/−) mice were ex vivo treated with phorbol 12-myristate 13-acetate (PMA, 200 ng/ml, positive control), organic dust extract (5% ODE), or media alone in submerged in vitro culture. PKCε activity was assayed from 1-24 hr. Results represent mean ± SEM (N=5-6 per condition) with statistical significance denoted by asterisks (**p<0.01, ***p<0.001) versus media alone, and hatch marks (###p<0.001) denotes difference between matched WT and KO conditions.