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. Author manuscript; available in PMC: 2014 Jun 23.
Published in final edited form as: Respir Physiol Neurobiol. 2012 Feb 2;181(1):99–108. doi: 10.1016/j.resp.2012.01.011

Figure 2. Determination of IF, ACh-elicited force, and total force generation.

Figure 2

Forceresting was initially set to 4.9 mN (0.5 g) in 2.39 mM Ca2+ buffer. Following buffer substitution with 0.0 mM Ca2+ buffer Forceresting decreased to a stable nadir value. IF was defined as the difference between initial force and the nadir value. Subsequently, the buffer was replaced with 2.39 mM Ca2+ buffer and forceresting was not significantly different than original. ACh 10-5 M was added (ACh-elicited force). Total force was the sum of IF and ACh-elicited force.