Fig. 4.
Enhanced SSC activity in Fbxw7 KO testis cells. (A) Experimental procedure. Testis cells from Fbxw7f/f mice were dissociated and incubated with AxCANCre overnight and then injected into the seminiferous tubules of W testes. (B) Macroscopic appearance of recipient testis. Histological appearance (Inset). (C) Colony counts after Cre transfection and transplantation of Fbxw7f/f mouse testes. Results of three experiments (n = 18). (D) RT-PCR analysis of spermatogenic gene expression in recipient testes. (E) Colony counts after Pin1 depletion and transplantation of green mouse testes. Results of two experiments (n = 16). (Scale bars: B, 1 mm; B, Inset, 50 μm.)