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. 2014 Jun 2;111(24):8889–8894. doi: 10.1073/pnas.1323649111

Fig. 1.

Fig. 1.

Reprogramming efficiency of NHEJ-deficient fibroblasts. (A) Human Artemis-, DNA-PKcs–, and LIG4-deficient fibroblasts were reprogrammed to iPSCs by lentiviral-mediated vector transduction. Reprogramming efficiency was calculated as the percentage of morphologically intact iPSC colonies obtained per total number of fibroblasts transduced. (B) Fibroblasts from given cell lines were either OKSM- or mock-transduced and sorted for dTomato expression after 48 h. Percentage of p53-expressing cells was assessed by immunofluorescence after 7 and 10 d of culture. (C) LIG4-deficient fibroblasts transduced with either human codon optimized LIG4 cDNA (coLIG4)-expressing vectors or with mock vectors were reprogrammed to iPSCs. Complementation of LIG4 partially rescued the reprogramming efficiency obtained in the previous experiments (*P ≤ 0.05). (D) The percentages of plasmids repaired by direct joining (DJ), microhomology-mediated joining (MH), combinations of MH and deletions (MH + del), or deletions (del) not associated with MH or insertion were analyzed in iPSCs transfected with the linearized pDVG94 construct and are reported for given cell lines (*P ≤ 0.05).