Fig. 4.

Differentiation efficiency and DNA damage response of myeloid cells derived from NHEJ-deficient iPSCs. (A) EBs were developed from human control, Artemis-, catalytically DNA-PK– and LIG4-deficient iPSCs. One representative experiment of five is shown. (B) EBs from the indicated cell lines were cultured in methylcellulose and granulocyte colonies (CFU-G) were picked after 14 d. (C) Granulocyte maturation was assessed based on nuclear and cytoplasmic morphology and divided into groups of promyelocytes, myelocytes/metamyelocytes, and bands/segmented cells (*P ≤ 0.05). (D) Myeloid cells (CFU-G, CFU-M, and CFU-GM) of indicated cell lines were irradiated with 2 Gy, and γH2AX foci formation is expressed as mean foci numbers per cell + SEM (*P ≤ 0.05).