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. 2014 Jun 4;111(24):8913–8918. doi: 10.1073/pnas.1323785111

Fig. 5.

Fig. 5.

TM6SF2 overexpression reduces lipid droplet content of human hepatoma Huh7 cells. Human hepatoma Huh7 cells were transfected with FP635-tagged TM6SF2 plasmids or FP635-tagged CALR (control) plasmids. Following 48-h incubation, cells were stained with BODIPY493/503 and analyzed by confocal microscopy. Representative fields of 20–30 cells transfected with FP635-tagged TM6SF2 plasmids (Upper) and FP635-tagged CALR plasmids (Lower) are shown. Note that in Upper, the lipid droplet content (green color) is considerably lower in TM6SF2 transfected cells (red color) compared with the nontransfected cell (absence of red color). In contrast, no difference in lipid droplet content is observed for the CALR transfected cells, as shown in Lower. (Scale bar, 50 μm.)