Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Jun 2;111(24):8979–8984. doi: 10.1073/pnas.1403731111

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Freely available online through the PNAS open access option.

PMC Copyright notice

Fig. 5. — Cell-surface localization of Ca_V2.2 and α₂δ-1 in DRG neurons. Cell-surface expression of Ca_V2.2-HA (A) and α₂δ-1 (B and C) in nonpermeabilized DRG neurons transfected with Ca_V2.2-HA/α₂δ-1/β1b and VAMP-mCherry. Transfected cells were identified by VAMP-mCherry (red). (Lower) Merged images). (A) Ca_V2.2-HA immunostaining (green); 58/71 mCherry-positive DRG examined (81.7%) had surface HA signal in this condition. (B) α₂δ-1 immunostaining (green); 0/20 mCherry-positive DRG had surface α₂δ-1 signal. (C) α₂δ-1 immunostaining (green) after antigen retrieval; 52/62 mCherry-positive DRG (85.5%) had surface α₂δ-1 signal in this condition. (Scale bar, 20 μm.) Representative of two separate transfections.