Fig. 1.

Time course of Nct inactivation in the hippocampus of Nct cKO mice. (A) Western analysis of protein levels of Nct at five different time points. Hippocampal lysates were treated with PNGase-F to deglycosylate Nct. Nct levels are reduced progressively in the Nct cKO hippocampus. (B) Hippocampal lysates from Nct cKO and control mice at age 30, 45, 60, 75, and 90 d were analyzed by immunoblotting. Protein levels were normalized to β-actin and measured by LI-COR quantitative detection system. All data represent means ± SEM. The number of mice used in the experiment is indicated in parentheses.