Fig. 3.

Clonal analysis reveals limited proliferation underlying postnatal cardiogenesis. (A) (Upper) Frequency of single-labeled cells at different time points after recent TM administration (n = 3 per group). (Lower) Analysis of individual-colored clone size for each time point above. (B, (Left) Two GFP⁺ cell clones; (Right) two RFP⁺ cell clones with borders outlined by wheat germ agglutinin staining (orange color). (Scale bars, 5 µm.) (C) Frequency of single-labeled cells 3 and 18 mo after P10 TM pulse reveals no significant proliferation of daughter cells. (Lower) Analysis of clone size for each time point above. N = total number of single-labeled cells observed per group. (P values calculated with Student t test. Error bars indicate SEM).