Skip to main content
. 2014 Jun 3;111(24):E2453–E2461. doi: 10.1073/pnas.1406459111

Fig. 3.

Fig. 3.

Nup85 is a poor substrate for BioID. IB analysis detects low levels of endogenous Nup85 (open arrowhead) in the Nup160, Nup107, and Nup43 BioID pull-down samples and significant levels of the exogenous mycBirA*-Nup85 (arrowheads) in the Nup85 BioID pull-down. (Top) For clarity, total lysates are shown at a lower exposure than the BioID samples. (Middle) For comparison, we detect similar levels of the BirA*-fusion proteins with anti-BirA in these same samples. (Bottom) Reprobing the same membrane with anti-Nup107 reveals levels of endogenous Nup107 (arrow) that correlate with the MS results, thus corroborating those results. Exogenous mycBirA*-Nup85 remains detected below the endogenous Nup107 (asterisk).