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. 2014 Jun 3;111(24):E2453–E2461. doi: 10.1073/pnas.1406459111

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Fig. 4. — Nup43 interacts with Nup85. (A) Anti-HA co-IP from lysates of HEK293 cells cotransfected with Nup43-HA (Middle, arrowhead) and GFP–Y-Nups (Top) indicates that GFP-Nup85 is pulled down most efficiently by Nup43-HA. (Bottom) Reprobing the samples with anti-Nup107 reveals low levels of endogenous Nup107 (arrow) in all the Nup43-HA pull-down samples. (B) Co-IPs from in vitro transcription/translation reactions in reticulocyte lysates using Nup43-HA (Lower, arrowhead) alone or with mycBirA*-tagged Y-Nups (Upper). Only mycBirA*-Nup85 is detected in the Nup43-HA pull-down fraction.