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. 2014 Jun 13;14:51. doi: 10.1186/1475-2867-14-51

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Copyright © 2014 Chlapek et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Changes in the expression of the GDF15 gene in Daoy (A) and D283 Med (B) cells analyzed using RT-PCR. The cells were treated with ATRA alone or in combination with CA (an inhibitor of 5-LOX) or CX (an inhibitor of COX-2); numbers indicate the concentrations of these compounds in μM. Respective numerical data from densitometry for three independent experiments are shown in the graphs: X-axis, treatment condition; Y-axis, relative gene expression. The data represent the means ± SD; they were normalized to the expression of the HSP90AB1 housekeeping gene and were related to the untreated control cells. Representative electrophoresis is presented for each cell line.