Skip to main content
. 2014 Jun 23;4:5405. doi: 10.1038/srep05405

Figure 2. Optimization of transfection conditions of CRISPR/Cas9 plasmid to inactivate GFP.

Figure 2

(A) 293-SC1 cells were transfected with different amounts of CRISPR/Cas9 plasmid. The cells were saturated with 1.5 μg of CRISPR/Cas9 plasmids. With 1.5 μg plasmids/well, approximately 51% of cells had inactivated GFP expression. (B) DNA sequence chromatograms of cells transfected with CRISPR/Cas9 are in a mass, compared with controls. (C) DNA sequence analysis of single GFP-negative colonies. WT, −3 bp, −87 bp represent wild-type, or with 3 bp deletion and 87 bp deletion of GFP gene, respectively.