Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Apr 29;42(11):6901–6920. doi: 10.1093/nar/gku312

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 4. — MYC stimulates histone H3 acetylation and inhibits H3K27 trimethylation but does not modulate H3K4 trimethylation. (A)–(F) P493-6 B cells were treated as described in the legend to Figure 3. ChIP was performed with antibodies against H3K4me3 (A), H3ac (B), H3K9ac (C), H3K27ac (D), H3K27me3 (E) and CBP/p300 (F). (G) Sequential ChIP was carried out by first immunoprecipitating MYC-containing complexes, followed by a second ChIP with antibodies against H3ac, H3K9ac, H3K27ac, H3K4me3, H3K27me3, or IgG control. The DNA fragments were amplified by qPCR with the primers indicated in the scheme. Error bars represent s.d. of PCR triplicates.