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. 2014 Apr 29;42(11):6901–6920. doi: 10.1093/nar/gku312

Figure 7.

Figure 7.

Binding of MYC and WDR5, a core subunit of ASH2L–KMT2 complexes, correlates with gene expression. (A) Proportion of ChIP-Seq peaks of WDR5, MYC and both MYC and WDR5 in comparison to distinct types of regulatory regions in NHEK cells. Chromatin regions were defined by the presence of ChIP-Seq peaks of distinct histone modifications or combinations thereof and the distance of the regions to transcriptional start sites. (B) The proportion of MYC (Jaspar Motif MA0147.1) and ASH2L (54) binding sites inside WDR5, MYC or MYC+WDR5 ChIP-Seq peaks. In all cases, the number of MYC and ASH2L-binding sites were significantly higher than binding sites in randomly selected genomic regions (*indicate conditions with a z-test p-value < 0.0001). (C) The log of the number of RNA reads per kilobase pairs on genomic regions close (+/- 500 bp) to WDR5, MYC and MYC+WDR5 ChIP-Seq peaks. We only considered those ChIP-Seq peaks overlapping with the 200 bp upstream regions of transcriptional start sites. (D) RNA-Seq-derived strand specific expression (red) and ChIP-Seq profiles (blue) and their corresponding peaks (green) of genomic regions around ODC, NCL and CCND2.