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. 2014 Apr 25;42(11):6921–6934. doi: 10.1093/nar/gku326

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 8. — (A) Coverage plots of RNA-seq data over the SMAD7 gene. The TGFβ stimulated (TGFB+) data have been normalized to the same sequencing depth as the unstimulated (TGFB−). (B) Scatter plot of RPKM-counts over genes for TGFB+ and TGFB−. The genes selected for Taqman validation are marked with black triangles. (C) Taqman validation results for 25 selected genes. The RNA-seq is represented as log2 of sequence depth normalized fold change between TGFB+ and TGFB−, and the Taqman values are also log2 of fold change between TGFB+ and TGFB−.