Figure 7.

Functional analysis of designer (d)Bat constructs targeting the human SOX2 promoter. dBats were tested using flow cytometry with a transcriptional activation reporter as described (Figure 3). Population curves for dsGFP fluorescence are shown (top) as well as boxplots of fluorescence intensities (bottom) compared to the reporter control (logarithmic scale). Boxplots show fold changes in fluorescence intensity compared to the reporter control with whiskers representing the 2.5% and 97.5% data limits. Median values are written next to each box plot and shown graphically as thick black lines. Two dBats, designed based on the RVD (dBat_{SOX2 RVD switch}) or repeat switch (dBat_{SOX2 repeat switch}), and an equivalent dTALE were tested.