Skip to main content
. 2014 May 9;42(11):7069–7083. doi: 10.1093/nar/gku333

Figure 2.

Figure 2.

Domains of Spt5 that interact with RNAP II. (A) Schematic showing different domains of the yeast Spt5. Spt5 residues that cross-link to Rpb1, Rpb2, Rpb4 and Rpb7 are shown below the schematic. (B–E) Western blots showing cross-linking of Bpa-substituted Spt5 to Rpb1, Rpb2, Rpb4 and Rpb7. Rpb1 was detected with antibody 8WG16. 3×Myc-tagged Rpb2, Rpb4 and Rpb7 were detected with an anti-Myc antibody. Sites of Bpa substitutions are shown above the lanes of each blot. Bands of cross-linked proteins are indicated with red asterisks. (F) Western blot showing cross-linking of Bpa-substituted Rpb7 to CTR-deleted Spt5 (Spt5ΔCTR). Spt5p stands for phosphorylated Spt5, which disappears following phosphatase treatment or deletion of Spt5 CTR or the BUR2 gene (23). 3×Myc-tagged Spt5 was detected with an anti-Myc antibody.