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. 2014 May 9;42(11):7069–7083. doi: 10.1093/nar/gku333

Figure 8.

Figure 8.

TFIIE and Spt5 interact with the clamp and Rpb4/7 stalk of RNAP II differently. (A and B) Western blots showing cross-linking of Bpa-substituted Rpb1 and Rpb7 to Tfa1, respectively. 3×Myc-tagged Tfa1 was detected with an anti-Myc antibody. Sites of Bpa substitutions are shown above the lanes of each blot. Bands of Tfa1 cross-linked to Bpa-substituted Rpb1 and Rpb7 are indicated with red asterisks. (C) Locations of Bpa-substituted Rpb1 and Rpb7 residues that cross-linked to Tfa1. Residues that cross-linked to Tfa1 are shown in black. Orange circle indicates Rpb7 residues that also cross-linked to Spt5 (see Figure 1D and E). The two Rpb1 residues (E291 and H281) that cross-linked to Spt5 (see Figure 1A and E) but not Tfa1 are shown in purple. The RNAP II structure is based on PDB 1Y1W (35).