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. 2014 May 3;42(11):7160–7169. doi: 10.1093/nar/gku341

Figure 1.

Figure 1.

Imperfect target sequences of AvrBs3 increase the importance of T0. (A) Schematic presentation of AvrBs3 and reporter constructs. Repeats are indicated by ovals [gray: degenerated repeats in the N-terminal region (NTR)]. T3S signal: T3S secretion signal; NLS: nuclear localization signal; AD: activation domain; EBE: effector binding element (upper strand); N0: position zero of EBE. Bold letters refer to mismatches in the EBEAvrBs3. EBEs were fused to the Bs4 minimal promoter (pBs4min) driving expression of the β-glucuronidase (GUS) reporter gene uidA. (B) Quantification of AvrBs3 activities for different EBEs. GUS activities were determined 3 days after Agrobacterium-mediated delivery of effector- and reporter-constructs into leaves of Nicotiana benthamiana (see ‘Materials and Methods’ section). Asterisks indicate a significant difference in activity of the same TALE-derivative tested with EBE-T0 (Student's t-test; *P-value ≤ 0.05; **P-value ≤ 0.01; ***P-value ≤ 0.001). Experiments were performed three times with similar results.