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. 2014 May 3;42(11):7160–7169. doi: 10.1093/nar/gku341

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — The number of AvrBs3 repeats affects T₀-dependency. (A and B) GUS activities induced by AvrBs3 and derivatives 3 days after Agrobacterium-mediated delivery of effector- and reporter-constructs into leaves of Nicotiana benthamiana. Asterisks indicate a significant difference in activity of the same TALE-derivative tested with EBE-T₀ (Student's t-test; *P-value ≤ 0.05; **P-value ≤ 0.01; ***P-value ≤ 0.001). (C) Comparison of relative GUS activities induced by AvrBs3-derivatives dependent on the base at position N₀. Constructs and corresponding EBEs are schematically presented in the right panel. AvrBs3 derivatives differ in number of repeats and the repeat 1 RVD (black oval; RVD = XX). Chosen RVD1: HD or NS. The activity of AvrBs3-derivatives for EBE(T₀) was set to 100%. Relative GUS values are based on the mean of three independent experiments. Color scale: GUS activities smaller than 100%.