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. 2014 May 16;42(11):7290–7304. doi: 10.1093/nar/gku347

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — SE contain a few abundant miRNAs. (A) Top 15 most highly expressed unique miRNAs in SE. Left axis and bars: percent of total miRNA reads that mapped to each of the top 15 most abundant miRNAs. Error bars are standard deviation. Right axis and line: cumulative percent of total miRNA reads. (B) ddPCR analysis of miRNAs. RNA was isolated from three additional SE samples that had not been sequenced and assayed for miRNA levels by ddPCR assay. The five highest abundant (by sequencing) and two low abundance miRNAs were tested. Counts per μl of input cDNA are shown for each of the three donors. All reactions were run in triplicate with extremely low standard deviations (error bars always fell within each symbol and are not shown for better visual clarity). miR28 was analyzed in two donors only.