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DprA−RecA interaction tests using Y2H assays. Yeasts expressing wild-type or mutant SpDprA as Gal4 binding domain fusion (BD-SpDprA) and variants of RecA as Gal4 activation domain fusions (AD-SpRecA) were spotted as a series of 1/5th dilutions on selective medium lacking histidine. Plates were incubated for 5 days at 28°C. The left column indicates the nature of DprA, the upper line defines the length of RecA and the right column shows its content in terms of mutations.
