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. 2014 May 13;42(11):7330–7345. doi: 10.1093/nar/gku357

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Gno1p is required for the normal accumulation of the free large ribosomal subunit. (A) Accumulation of free ribosomal subunits and polysomes in Δgno1compared to WT cells. Extracts from wild type (WT) (left panel) and Δgno1 (right panel) cells grown in yeast extract peptone (YP) glucose medium were centrifuged on 10–50% sucrose gradients at 39 000 revolutions per minute in a SW41Ti rotor. The abundance of free 40S, 60S ribosomal subunits, 80S ribosomes and polysomes was assessed by recording the ultraviolet absorbance at 254 nm in the successive gradient fractions. (B) Accumulation of free ribosomal subunits and polysomes in Δgno1 cells expressing Gno1p-HA (bottom left panel) or PINX1-HA (bottom right panel). As in (A), except that cells were grown in supplemented yeast nitrogen medium (YNB Glu). Profiles obtained with WT (upper left panel) and Δgno1 cells (upper right panel) are shown as controls.