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. 2014 May 13;42(11):7330–7345. doi: 10.1093/nar/gku357

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Consequences of alterations to the G-patch domain of Gno1p or PINX1 on ribosome biogenesis in yeast. (A) Northern analysis of rRNAs and pre-rRNAs extracted from Δgno1 cells expressing Gno1p or PINX1 variants featuring amino acid substitutions within their G-patch domain. Total RNAs were extracted from Δgno1 cells transformed with yeast vectors directing expression of wild-type Gno1p-HA (lane 1), Gno1pGm1-HA (lane 2), Gno1pGm2-HA (lane 3), PINX1-HA (lane 4), PINX1Gm1-HA (lane 5), PINX1Gm2-HA (lane 6) or the empty parental vector (lane 7) and analyzed by northern as described in the legend of Figure 4A. (B) Northern analysis of pre-rRNAs co-precipitated with HA-tagged Gno1p, PINX1 or G-patch variants thereof. Immunoprecipitation experiments were performed with an anti-HA matrix using extracts from Δgno1 cells expressing the indicated HA-tagged protein or no tagged protein as control. Input (lanes 1 to 7) and co-precipitated (lanes 8 to 14) pre-rRNAs were analyzed as described in the legend of Figure 5A.