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. 2014 May 16;42(11):7473–7485. doi: 10.1093/nar/gku402

Figure 7.

Figure 7.

Paired Cas9 nickases with one bulge-containing sgRNA effectively cleave genomic DNA. (A) Human HBB gene targeted by Cas9 nickases (Cas9n) with paired guide strands R-01 and R-02. PAMs are indicated with grey bars. (B) T7E1 activities of Cas9n with R-01 bulge-variants paired with R-02, compared with original Cas9 activities of the R-01 bulge-variants as in Figures 2 and 4. Error bar, SEM (n = 2). Asterisks indicate P-values from a two-tailed independent two-sample t-test. *P < 0.05, **P < 0.01, ***P < 0.001.