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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Mol Cell Neurosci. 2014 Mar 27;60:53–62. doi: 10.1016/j.mcn.2014.03.002

Figure 5. Syntaxin 3B is associated with the synaptic plasma membrane.

Figure 5

Subcellular fractionation of mouse retina was performed to analyze the distribution of syntaxin 3B. Synaptosomes (P2) were isolated from mouse retina by differential centrifugation. The synaptosomes were then hypo-osmotically lysed and separated into synaptic plasma membrane fraction (LP1), synaptic vesicle fraction (LP2) and soluble presynaptic protein fractions (LS1 and LS2). Equal protein amounts were analyzed by SDS-PAGE and western blotting using specific antibodies directed against SV2B (ribbon synaptic vesicle marker), syntaxin 3, SNAP-25 and PSD-95 (postsynaptic density protein).