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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Mol Cell Neurosci. 2014 Mar 27;60:53–62. doi: 10.1016/j.mcn.2014.03.002

Figure 6. The N-terminal domain reduces binding of syntaxin 3B to SNAP25.

Figure 6

SNAP-25 and Munc18 binding to GST fusion proteins of syntaxins 1A and 3B without transmembrane domains and the isolated syntaxin 3B SNARE domain was analyzed using pulldown assays with retina extract followed by SDS-PAGE and western blotting using SNAP-25 antibodies and GST antibodies (loading control). The structure of the recombinant proteins is depicted below. Two different amounts of GST-syntaxin 3B protein were loaded to allow a better comparison. Recombinant GST fusion proteins of the predicted size are marked with arrows.