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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Mol Cell Neurosci. 2014 Mar 27;60:53–62. doi: 10.1016/j.mcn.2014.03.002

Figure 7. Syntaxin 3B is phosphorylated by CaMKII at T14.

Figure 7

A. GST-syntaxin fusion proteins were in vitro phosphorylated by CaMKII and Ɣ -32P ATP, separated by SDS-PAGE and analyzed by autoradiography (32P) and immunoblotting with GST antibodies. Two different amounts of syntaxin 3B were loaded to allow better comparison with the other proteins.

B: Wildtype GST-syntaxin 3B as well as mutated proteins were phosphorylated and analyzed as in A. Wild type syntaxin 3B and mutant proteins GST-syntaxin 3B T81A, S145A, and S187A were phosphorylated similarly, while the mutant protein GST-syntaxin 3B T14A was not phosphorylated, demonstrating that T14 is the phosphorylation site in syntaxin 3B.