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. 2014 May 29;3:e02811. doi: 10.7554/eLife.02811

Figure 2. TAF7L bidirectionally regulates BAT/muscle lineages.

(A) 2 days (2D) and 5 days (5D) post-induced control (shCNTL) and TAF7L knockdown (shTAF7L) C3H10T1/2 cells stained with Oil Red O. (B) mRNA levels of myoblast genes Myf5, Myod1, Mef2c, Myhc, and Mlc in 2D post-induced shCNTL and shTAF7L C3H10T1/2 cells, compared to C2C12-induced myotubes. (C) Protein levels of TAF7L, PPARγ, and UCP1 in 5D post-induced shCNTL and shTAF7L cells; Pol II was used as loading control. (D) mRNA levels of brown fat genes (Pgc1a, Ucp1, and Cidea) in 5D post-induced control and shTAF7L cells. (E) 5D post-induced control (C2C12.CNTL) and TAF7L-expressing C2C12 (C2C12.TAF7L) cells were stained with Oil Red O. (F) mRNA levels of myoblast genes Myf5 and Myod1 in pre-induction cells. (G) mRNA levels of BAT-specific genes (Ucp1, Cidea, Cox8b, Pgc1a, and Pparα) 5D post-differentiation. *p<0.05, data is mean and SEM is from triplicates.

DOI: http://dx.doi.org/10.7554/eLife.02811.006

Figure 2.

Figure 2—figure supplement 1. TAF7L is required for brown fat cell differentiation from primary brown adipocytes.

Figure 2—figure supplement 1.

(A) Isolated brown adipose progenitor cells from WT (TAF7L+/Y) and Taf7l KO (TAF7L-/Y) mice was induced with brown adipocyte differentiation regime for 5 days and then stained with Oil Red O. (B and C) mRNA levels of fat-selective genes (B) and muscle-selective genes (C) pre-differentiation. *p<0.05, data is mean and SEM is from triplicates. (D) Expression levels of Taf7l and brown adipocyte marker genes on cells from A, the expression levels of genes in 5D post-induced Taf7l knockout cells were compared to WT cells, whose levels were assigned to 1.
DOI: http://dx.doi.org/10.7554/eLife.02811.007

Figure 2—figure supplement 2. Gene expression analysis of control (CNTL) and TAF7L-expressing (TAF7L) C2C12 cells from 0 (0D) to 5 days (5D) post BAT-induction.

Figure 2—figure supplement 2.

mRNA levels of Ucp1, Prdm16, Cidea, Pgc1a, Taf7l, and Myod1 are plotted as relative value to 0D CNTL cells, data is mean and SEM is from triplicates.
DOI: http://dx.doi.org/10.7554/eLife.02811.008