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. 2014 Jun 3;5:20. doi: 10.1186/2041-9139-5-20

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Copyright © 2014 Li et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Expression of BfNpas4.In situ hybridization of BfNpas4 with antisense probe. (A) No expression is detected in mid-neurula with seven somites. (B,C) In the nine-somite late neurula, BfNpas4 is expressed in two regions; the left region (arrows) is slightly anterior to the right region (arrowhead). (D,E) This expression pattern is maintained in the late neurula with 12 somites, and the distance between the two regions increases. The scale bar applies to all panels. Anterior is to the left. A, B, and D are lateral views; C and E are dorsal (d) views.