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. 2014 Jun 3;5:20. doi: 10.1186/2041-9139-5-20

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Copyright © 2014 Li et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Expression of BfHifα.In situ hybridization of BfHifα with antisense probe and with sense probe. (A-H) From blastula to mid-neurula, BfHifα is ubiquitously expressed at a low level. (I,J) In two-day larvae, localized expression is found in the cerebral vesicle (arrow). (K,L) Cryosections of an amphioxus juvenile show BfHifα expression in the central nervous system (arrows), the pharyngeal bars (arrowheads), and the intestine (open arrowhead). The scale bar in A applies to A-J. Blastoporal views are labeled as ‘bp’, and panels E-J are lateral views; ‘d’ denotes the dorsal side of the larva in I. In E-H, anterior is to the left; in I and J, anterior is to the upper left.