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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Stem Cell Res. 2013 Jul 16;11(3):1013–1021. doi: 10.1016/j.scr.2013.07.001

Figure 4.

Figure 4

HAECs rescue functional hematopoiesis up to 48 h following radiation injury. (A) BMC harvested from 580 cGy-irradiated mice were initially cultured for 24 h or 48 h in control conditions and then cultured in either the presence or absence of HAECs for 7 additional days. (B) Absolute CD150+LSK cells (per 2 × 106 input BMC) recovered after a 48 h post-irradiation delay and 7 days of culture (n = 5 independent experiments). (C) CFU activity (per 2 × 106 input BMC) after a 24 h or 48 h post-irradiation delay (n = 5–7 independent experiments). (D) PB engraftment by 48 h HAEC-rescued BMC for up to 30 weeks following transplantation (n = 5 recipients/group). BMC cultured in the absence of ECs provided no measurable level of engraftment (not detectable, N.D., sensitivity = 0.5%). (E) 30 week multilineage hematopoietic reconstitution of the PB by HAEC-rescued BMC after a 48 h delay. Error bars show SEM.