Figure 5.
Silencing of HBV replication in vivo and reporter gene expression following administration of HD Ads expressing anti-HBV pri-miR mimics from the MTTR promoter. Mice were infected with a single dose of 5 × 109 infectious HD Ad particles expressing pri-miR-122/5, pri-miR-31/5 and pri-miR-31/5-8-9. Animals injected with saline or HD Ad Δ28 served as negative controls. (a) Time course of serum HBsAg concentrations, as measured by ELISA, following HD Ad administration. (b) Circulating viral particles (VPEs) were measured using Q-PCR following injection of HBV transgenic mice with HD Ads. Data are expressed as means with SEMs from each group of six mice. The statistical significance was calculated using a pair-wise comparison according to the Student t-test. P values less than 0.05 (∗) or less than 0.01 (∗∗) were considered statistically significant.