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. Author manuscript; available in PMC: 2014 Jun 23.
Published in final edited form as: Biochem Biophys Res Commun. 2011 Nov 19;417(1):49–55. doi: 10.1016/j.bbrc.2011.11.048

Fig. 3.

Fig. 3

EGFR and Src inhibitors fail to block decreased Cof-P and increased cell migration in response to smoke when cells lack p120ctn. (A–D) p120WT and p120KD cells were treated with smoke-free (Ctrl) or smoke medium at 48 mg/m3 TSP (Smk) for 3 h in the presence of 1 μM AG1478 (A and B) or 12.5 μM PP2 (C and D) to inhibit EGFR or Src, respectively. Western blot (A and C) and densitometric analysis (B and D) of Cof-P in Smk-treated cells were normalized to Ctrl (designated 1-fold). Data are expressed as the mean ± SEM of three independent experiments. (E) P120WT and p120KD cells were loaded on Boyden chambers and incubated with Ctrl (white bar) or smoke medium at 48 mg/m3 TSP (black bar) in the presence of DMSO (vehicle control) or 12.5 μM PP2 for 4 h. Migrating cells were quantified by measuring the OD at 595 nm and plotted as the mean ± SEM of three independent chambers. **p < 0.01, smoke-treated p120WT or p120KD cells versus respective control. **p < 0.01, smoke-treated p120KD cells versus smoke-treated p120WT cells.