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. Author manuscript; available in PMC: 2015 Apr 8.
Published in final edited form as: Cells Tissues Organs. 2014 Apr 8;198(6):438–447. doi: 10.1159/000360275

Fig. 4.

Fig. 4

Effect of exogenous BMP6 on osteogenesis of DFSCs. (a) Note that addition of hrBMP6 to the osteogenic induction medium resulted in no obvious effect on osteogenesis in passage 3 (P3) DFSCs (upper panel), but dramatically increased osteogenesis in passages 7 (P7) and 11 (P11) DFSCs (middle and lower right panel) as shown by Alizarin Red staining of calcium deposits. (b) Quantitation of osteogenesis by analyzing the Alizarin Red staining using Image-Pro Analyzer. Statistical analysis of the results showed a significant increase of osteogenesis by adding hrBMP6 to the medium for osteogenic induction of P7 and P11 DFSCs. Double asterisks (**) indicate a significant difference at P≤0.01 (n=3). (c) Expression of BSP and Runx2 in the passage 7 DFSCs after 2 weeks of osteogenic induction as determined by real-time RT-PCR. Increased expression of BSP and Runx2 were detected in the DFSCs when BMP6 was added to the induction medium. Asterisk (*) indicates a significant difference at P≤0.05 (n=3).