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. Author manuscript; available in PMC: 2015 Apr 8.
Published in final edited form as: Cells Tissues Organs. 2014 Apr 8;198(6):438–447. doi: 10.1159/000360275

Fig. 5.

Fig. 5

Effect of BMP6 knockdown on osteogenesis of DFSCs. (a) BMP6 expression was greatly knocked down by BMP6-siRNA as determined by Western blotting. (b) Knockdown of BMP6 reduced osteogenic differentiation capability of DFSCs as seen by comparing the BMP6-siRNA transfected DFSCs to the controls (untransfected and scrambled siRNA transfected DFSCs). Incubation of BMP6-siRNA transfected cells in osteogenic medium containing hrBMP6 protein restored the osteogenesis (lower right panel). (c) Osteogenesis was quantified by analyzing the Alizarin Red staining using Image-Pro Analyzer. Statistical analysis indicated significant reduction of osteogenesis after BMP6 knockdown, and addition of BMP6 could significantly restore osteogenesis (n=4). Bars labeled with a same letter indicate no significant difference at P≤0.05.