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. 2014 May 23;48(12):7062–7068. doi: 10.1021/es5019948

Figure 1.

Figure 1

Effects of combined exposure of SeMet (50 μM) and hypersaline conditions on the development and hatchability of Japanese medaka embryos after exposure at 12 hpf. White bars represent freshwater and black bars represent saltwater. (A) Percent hatch, (B) median day to hatch, (C) percent deformities in hatched embryos, (D) examples of deformities; control is top image, bottom two images demonstrate lordosis, kyphosis, cranio-facial abnormalities, and yolk-sac edema. Each value represents the mean ± standard error (SE) of 5–10 replicates. Statistical significance is indicated by differing letters (Two-way ANOVA, Tukey HSD test, or Kruskal–Wallis, Dunn’s test p ≤ 0.05).