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. 2014 May 23;48(12):7062–7068. doi: 10.1021/es5019948

Figure 4.

Figure 4

Effects of combined exposure of SeMet (50 μM) and hypersaline conditions on BiP, ATF6, and ATF4 transcripts in Japanese medaka embryos after 12 and 24 hpf. (A) Expression of BiP, ATF6, and ATF4 in whole embryos at 12hpf and 24hpf in freshwater. Expression of (B) BiP, (C) ATF6, and (D) ATF4 in 24hpf control embryos and SeMet treated embryos in freshwater and saltwater. Each value represents the mean ± SE of 5–10 replicates. EF1-α was run as a housekeeping gene. Statistical significance is indicated by differing letters (Two-way ANOVA, Tukey HSD Test p ≤ 0.05).